Modified gel electrophoresis for higer resolution of DNA fingerprints

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Modified gel electrophoresis for higher resolution of DNA fingerprints.

Pig-) The amount of polymorphic frag| merits in a DNA fingerprint d e ^ |-f I 1» pends on characteristics of the 0.8* individual's genome, restriction enzymes and minisatellite B [U l I probes used. In addition h ow0-8* i.»% ever, a high resolution of the DNA fragments during gel electrophoresis is essential. Therefore the following simple method was applied: Instead of using an agarose gel of ...

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Typing of Methicillin Resistant Staphylococcus Aureus Using DNA Fingerprints by Pulsed-field Gel Electrophoresis

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Pulsed-field gel electrophoresis of circular DNA.

Mobility of supercoiled (form I) and nicked circular (form II) plasmid DNAs was determined on two major forms of pulsed-field electrophoresis, CHEF and OFAGE. Plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with Saccharomyces cerevisiae chromosomes as standards. Agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels re...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1988

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/16.15.7739